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human embryonic kidney epithelial cells (hek-293t)  (iCell Bioscience Inc)

 
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    iCell Bioscience Inc human embryonic kidney epithelial cells (hek-293t)
    Human Embryonic Kidney Epithelial Cells (Hek 293t), supplied by iCell Bioscience Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic kidney epithelial cells (hek-293t)/product/iCell Bioscience Inc
    Average 90 stars, based on 1 article reviews
    human embryonic kidney epithelial cells (hek-293t) - by Bioz Stars, 2026-02
    90/100 stars

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    Related to . ( A ) Immunofluorescence study showing colocalization of BG4 (antibody that binds to G4 DNA) and mitochondrial genome. Nucleus from HeLa cells is stained with DAPI (blue color). Mitochondria are stained with MitoTracker green FM and G4 DNA with BG4 (Alexa-Fluor 568), a merged image is shown. A merge of red and green is depicted by Coste’s mask (colocalization is represented as a white dot). The experiments were done independently in HeLa and <t>HEK</t> <t>293T</t> cells and the quantitation of colocalization of BG4 with MitoTracker is shown in . ( B ) Increasing concentrations (0.2, 0.5, 1, and 2 µg) of purified BG4 was incubated with G-rich ( G1 ) and C-rich ( C1 ) oligomers and products were resolved on 5% native polyacrylamide gel. In each case a lane without protein (lane 1 and 6) served as control. The substrate and the bound complex are marked.
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    Related to . ( A ) Immunofluorescence study showing colocalization of BG4 (antibody that binds to G4 DNA) and mitochondrial genome. Nucleus from HeLa cells is stained with DAPI (blue color). Mitochondria are stained with MitoTracker green FM and G4 DNA with BG4 (Alexa-Fluor 568), a merged image is shown. A merge of red and green is depicted by Coste’s mask (colocalization is represented as a white dot). The experiments were done independently in HeLa and <t>HEK</t> <t>293T</t> cells and the quantitation of colocalization of BG4 with MitoTracker is shown in . ( B ) Increasing concentrations (0.2, 0.5, 1, and 2 µg) of purified BG4 was incubated with G-rich ( G1 ) and C-rich ( C1 ) oligomers and products were resolved on 5% native polyacrylamide gel. In each case a lane without protein (lane 1 and 6) served as control. The substrate and the bound complex are marked.
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    Image Search Results


    Related to . ( A ) Immunofluorescence study showing colocalization of BG4 (antibody that binds to G4 DNA) and mitochondrial genome. Nucleus from HeLa cells is stained with DAPI (blue color). Mitochondria are stained with MitoTracker green FM and G4 DNA with BG4 (Alexa-Fluor 568), a merged image is shown. A merge of red and green is depicted by Coste’s mask (colocalization is represented as a white dot). The experiments were done independently in HeLa and HEK 293T cells and the quantitation of colocalization of BG4 with MitoTracker is shown in . ( B ) Increasing concentrations (0.2, 0.5, 1, and 2 µg) of purified BG4 was incubated with G-rich ( G1 ) and C-rich ( C1 ) oligomers and products were resolved on 5% native polyacrylamide gel. In each case a lane without protein (lane 1 and 6) served as control. The substrate and the bound complex are marked.

    Journal: eLife

    Article Title: Unleashing a novel function of Endonuclease G in mitochondrial genome instability

    doi: 10.7554/eLife.69916

    Figure Lengend Snippet: Related to . ( A ) Immunofluorescence study showing colocalization of BG4 (antibody that binds to G4 DNA) and mitochondrial genome. Nucleus from HeLa cells is stained with DAPI (blue color). Mitochondria are stained with MitoTracker green FM and G4 DNA with BG4 (Alexa-Fluor 568), a merged image is shown. A merge of red and green is depicted by Coste’s mask (colocalization is represented as a white dot). The experiments were done independently in HeLa and HEK 293T cells and the quantitation of colocalization of BG4 with MitoTracker is shown in . ( B ) Increasing concentrations (0.2, 0.5, 1, and 2 µg) of purified BG4 was incubated with G-rich ( G1 ) and C-rich ( C1 ) oligomers and products were resolved on 5% native polyacrylamide gel. In each case a lane without protein (lane 1 and 6) served as control. The substrate and the bound complex are marked.

    Article Snippet: HeLa (human cervical cancer), HCT116 (human colon cancer), MEF (mouse embryonic fibroblast) and HEK 293T (human embryonic kidney epithelial cell line) were purchased from National Centre for Cell Science, Pune, India.

    Techniques: Immunofluorescence, Staining, Quantitation Assay, Purification, Incubation, Control